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J P Advis J P Advis
Professor
 Animal Sciences
Bartlett 213
84 Lipman Drive
New Brunswick, NJ 08901
Phone: 732-932-9240
Fax: 732-932-6996
Email: advis@aesop.rutgers.edu
Website: www.anisci.rutgers.edu/faculty/advis2/juan-p-advis.html

Expertise Summary
  To assess if the hypothalamic median eminence (ME) is a neuroendocrine control site where final hypophysiotropic release leading to the preovulatory surge of Luteinizing Hormone (LH), an obligatory event for reproduction to occur, might be ultimately regulated. We have used two animal models for these studies: hens, showing daily LH preovulatory surges for 15-25 days and ewes, showing an LH preovulatory surge at the end of each follicular phase in a 16-day estrous cycle.

In our studies, we have concentrated in assessing the hypothalamic control of LHRH by three of its ME putative inputs: ß-endorphin (ßEND), dopamine (DA), and neuropeptide Y (NPY). Through our immunocytochemical studies we mapped the hypothalamic distribution of LHRH and these three ME putative inputs, in hens and ewes. On the other hand, in our release studies, we used in vitro incubations of ME tissue and push-pull cannula (PPC) sampling of in vivo neuropeptide release from discrete areas of the ME.

In addition to our release studies, we assessed the possibility that enzymatic degradation of LHRH in the ME might be a regulated event of some physiological significance. Furthermore, we recently determined steady-state levels of mRNA and expression of c-fos antigens, in some of these putative inputs to ME-LHRH neuronal terminals. In addition, we have developed an extremely sensitive capillary electrophoresis-based assay, that uses fluorescence microscopy detection of derivatized samples, for the determination of multiple neuropeptides in ME-PPC perfusate samples.

Research Projects
  • STRESS AND MULTIPLE BRAIN NEUROPEPTIDES: IN VIVO RELEASE AND PEPTIDE BIOSYNTHESIS
  • The effect of diet and metabolic disrupters on brain proteins and peptides: A proteomic approach to the regulation of food intake neuropeptides

    • Selected Publications
    Effects of ethanol on secretion of hypothalamic neuropeptides regulating preovulatory LH release in sheep.
      Journal: Annual meeting of the Research Society on Alcoholism (RSA)
     Authors: Advis JP, Klein J and Sarkar DK
    Ethanol reduces the effect of hypothalamic ß-endorphin on natural killer cell activity.
      Journal: Annual Meeting of the Endocrine Society
     Authors: Boyadjieva N, Advis JP, and Sarkar DK

    Services/Activities
    Ad hoc reviewer for grants submitted to USDA
      Start Date: Jan 1 1999 12:00AM
     End Date: Jan 1 2004 12:00AM
      Description:
    Editorial Board Member of The Journal of Capillary Electrophoresis
      Start Date: Jan 1 1998 12:00AM
     End Date: Jan 1 2007 12:00AM
      Description:

     

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