APPROACH: Effects of alcohol and estrogen alone or together on prolactin-secreting lactotropic cell growth and proliferation will be investigated both in vivo and in vitro using bromodeoxyuridine assay. Hormone levels will be measured by radioimmuno assays. The activity of the cell regulatory gene transforming growth factor and its receptors will be determined by a combination of techniques including immunocytochemistry, in situ hybridization assay, RT-PCR, transfection assay and RNase protection assay. Animala will be given alcohol in liquid diet and estrogen by silastic implant under skin. In vivo studies will involve pituitary cell cultures and tumor cell lines that will be treated with alcohol with or without estrogen.