ALCOHOL AND HYPERPROLACTINEMIA
Project Number 06525 Starting 02/01/2003 Ending 03/31/2009
OBJECTIVES: To understand at cellular and molecular levels how alcohol and estrogen induce prolactinomas using the laboratory Fischer 344 rat as an animal model.
APPROACH: Effects of alcohol and estrogen alone or together on prolactin-secreting lactotropic cell growth and proliferation will be investigated both in vivo and in vitro using bromodeoxyuridine assay. Hormone levels will be measured by radioimmuno assays. The activity of the cell regulatory gene transforming growth factor and its receptors will be determined by a combination of techniques including immunocytochemistry, in situ hybridization assay, RT-PCR, transfection assay and RNase protection assay. Animala will be given alcohol in liquid diet and estrogen by silastic implant under skin. In vivo studies will involve pituitary cell cultures and tumor cell lines that will be treated with alcohol with or without estrogen.
These data provide first evidence that chronic ethanol consumption promotes development of pituitary tumors by affecting estrogen-responsive growth-regulating polypeptides.